产品名称：Accelagen/TurboBiotinylation DTTM Kit/50X TurboBiotinylase DT (0.1 mg)
10X TurboBiotinylation Mix (1 ml)/B0112P

产品编号：B0112P

市场价：¥5960.00

场地：美国(厂家直采)

产品分类：蛋白类>多肽>多肽合成>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$298.00

品牌： Accelagen

公司：Accelagen Inc

公司分类：

商品介绍

Accelagen TurboBiotinylation DT Kit, the best biotinylation kit

Protein PurificationBaculovirus ExpressionMammalian ExpressionE. coli ExpressionMolecular BiologyProducts

Products: Eznyme Toolbox: TurboBiotinylation DT Kit

TurboBiotinylation DT^TM Kit

Catalog #:	Size:	Concentration:	Price:
B0112P	50X TurboBiotinylase DT (0.1 mg) 10X TurboBiotinylation Mix (1 ml)	5 mg/ml	$298
B0112M	50X TurboBiotinylase DT (1 mg) 10X TurboBiotinylation Mix (1 ml)	5 mg/ml	$1,678
B0112L	50X TurboBiotinylase DT (5 mg) 10X TurboBiotinylation Mix (5 ml)	5 mg/ml	$5,400
T0112B	Bulk	5 mg/ml	Inquiry

To Order:

Please email PO to order@accelagen.com or fax PO to 888-239-6490

PRODUCT DETAIL

DATA SHEET

PROTOCOL

Components and Formulation:

50X TurboBiotinylase DT at 5 mg/ml (80 µM) in Storage Buffer of 50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 2 mM TCEP, 50% glycerol.
10X TurboBiotinylation Mix (50 mM Tris-HCl, pH 7.5, 100 mM MgCl₂, 100 mM ATP, 5 mM biotin)

TurboBiotinylase Purity:

TurboBiotinylase DT is a stable ultra-pure recombinant E. coli BirA biotin ligase with dual GST- and His-tags. It is purified from E. coli and has a calculated molecular weight of 62.4 kDa. It is free of protease or nuclease contamination. It can be easily removed by affinity capture through its GST- or His-tag.

TurboBiotinylase Activity:

The efficiency of biotinylation can be measured by mass spectrometry. Biotinylation adds 226.31 Da to a protein.

Alternatively, biotinylation can be evaluated by streptavidin-agarose pull-down. A Coomassie stained gel will visually show the efficiency of biotinylation.

Remove biotin and ATP from the biotinylation reaction sample by a spin desalting column.
Save sample as Total (T)
Incubate with streptavidin-agarose for 1 hour with agitation.
Spin down the streptavidin-agarose.
Save the supernatant as Unbound (U)
Save the streptavidin-agarose as Bound (B)
Run gel and stain with Coomassie Blue.

品牌介绍

Accelagen常用产品报价单

产品名	货号	公司分类	商城分类	美金价
Accelagen/TurboLigation Kit/L0110S/ 100 reactions	L0110S	Enzyme Toolbox	蛋白酶类	546.00
Accelagen/PAK1KD (recombinant human PAK1 kinase domain)/K0104S/ 10 µg	K0104S	Kinases	激酶	360.00