产品名称：Accelagen/PAK2KD (recombinant human PAK2 kinase domain)/100 µg/K0105M

产品编号：K0105M

市场价：¥27500.00

场地：美国(厂家直采)

产品分类：蛋白类>酶>激酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1375.00

品牌： Accelagen

公司：Accelagen Inc

公司分类：

商品介绍

PAK2KD, Human PAK2 Kinase Domain

Protein PurificationBaculovirus ExpressionE. coli ExpressionMolecular BiologyProducts

Products: Kinases: PAK2KD

PAK2KD (recombinant human PAK2 kinase domain)

Catalog #:	Size:	Concentration:	Price:
K0105S	10 µg	1 mg/ml	$375
K0105M	100 µg	1 mg/ml	$1,375
K0105L	1 mg	1 mg/ml	$5,640
K0105B	Bulk	1 mg/ml	Inquiry

To Order:

Please email PO to order@accelagen.com or fax PO to 888-239-6490

PRODUCT DETAIL

DATA SHEET

Sequence: GPHMTDEEIMEKLRTIVSIGDPKKKYTRYEKIGQGASGTVFTATDVALGQEVAIKQINLQKQPKKELIINEILVMKE LKNPNIVNFLDSYLVGDELFVVMEYLAGGSLTDVVTETCMDEAQIAAVCRECLQALEFLHANQVIHRDIKSDNVLL GMEGSVKLTDFGFCAQITPEQSKRSTMVGTPYWMAPEVVTRKAYGPKVDIWSLGIMAIEMVEGEPPYLNENPLRA LYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDVEKRGSAKELLQHPFLKLAKPLSSLTPLIMAAKEAMKSNR

The first 4 residues GPHM are from Turbo3C cleavage site.
Phosphorylated at T402.

Component and Formulation:

PAK2KD: 1 mg/ml in Storage Buffer of 25 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% glycerol, 5 mM DTT.

Activity:

Specific activity:85,806 pmoles/min/µg

Analysis of enzymatic activity was performed according to the Zlyte assay protocol (Invitrogen):

Different concentrations of PAK2KD were incubated in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl₂, 1 mM EGTA, 200 mM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
Developer solution was added to the reaction and the reaction was stopped after 1 hour of incubation at room temperature.
Fluorescence was detected using lexc=460±40 nm and lem=528±20 nm filters.

SDS-PAGE Analysis:

品牌介绍

Accelagen常用产品报价单

产品名	货号	公司分类	商城分类	美金价
Accelagen/TurboLigation Kit/L0110S/ 100 reactions	L0110S	Enzyme Toolbox	蛋白酶类	546.00
Accelagen/PAK1KD (recombinant human PAK1 kinase domain)/K0104S/ 10 µg	K0104S	Kinases	激酶	360.00